Subsidiaries*

Place of Incorporation

ASP Isotopes Guernsey Limited (formerly, PDS-Photonica Holdings (Guernsey) Limited)

Guernsey

ASP Isotopes South Africa (Proprietary) Limited (formerly, PDS Photonica Holdings South Africa (PTY) Limited)

South Africa

Enlightened Isotopes (Pty) Ltd**

South Africa

ASPI South Africa Asset Finance

South Africa

Enriched Energy LLC

Delaware, U.S.

ASP Isotopes UK Ltd

England & Wales

ASP Isotopes Services (UK) Limited

England & Wales

Enlightened Isotopes (Pty) Ltd

South Africa

ASP Isotopes ehf

Iceland

Quantum Leap Energy LLC

Delaware, U.S.

Quantum Leap Energy Limited

England & Wales

Quantum Leap Energy (Pty) Ltd

South Africa

QLE TP Funding SPE LLC

Delaware, U.S.

K2025267858 (South Africa) (Pty) Ltd

South Africa

Global Radiopharmacies LLC

Delaware, U.S.

East Coast Nuclear Pharmacy LLC

Florida, U.S.

NuMed Diagnostics, LLC**

South Carolina, U.S.

PET Labs Pharmaceuticals (Pty) Ltd**

South Africa

PET Labs Global Nuclear Medicine SEZC

Cayman Islands

Renergen Limited

South Africa

Tetra4 Proprietary Limited**

South Africa

Cryovation Proprietary Limited

South Africa

Renergen US Inc.

Delaware, U.S.

Skyline Builders Group Holding Limited**

Hong Kong

*

Please note that this list includes all subsidiaries of ASP Isotopes Inc. without regard to whether they would constitute a "significant subsidiary" pursuant to Item 601(b)(21)(ii) of Regulation S-K.

*

Not a wholly-owned direct or indirect subsidiary of ASP Isotopes Inc.